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International Journal of Latest Research in Science and Technology

DOI:10.29111/ijlrst   ISRA Impact Factor:3.35

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SILYMARIN INDUCES GLUCAGON LIKE PEPTIDE-1 SECRETION AND INCREASES ITS RECEPTOR GENE EXPRESSION IN PANCREATIC TISSUE IN PARTIALLY PANCREATECTOMIZED RATS

Research Paper Open Access

International Journal of Latest Research in Science and Technology Vol.6 Issue 3, pp 13-19,Year 2017

SILYMARIN INDUCES GLUCAGON LIKE PEPTIDE-1 SECRETION AND INCREASES ITS RECEPTOR GENE EXPRESSION IN PANCREATIC TISSUE IN PARTIALLY PANCREATECTOMIZED RATS

Claudia Soto, Imelda Gonz?lez, Luis Raya, Ulises Carrasco, Luz Tovar

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Received : 29 April 2017; Accepted : 20 May 2017 ; Published : 30 June 2017

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Abstract

Glucagon-like peptide-1 (GLP-1) is an incretin produced and released by gut endocrine L-cells in response to the ingestion of carbohydrates, lipids and proteins. It is also produced in the pancreatic tissue, both in humans and rats. In the pancreatic tissue, GLP-1, stimulates insulin secretion in β-cells, induces neogenesis, proliferation, differentiation and maintenance of β cell mass, and inhibits apoptosis of these cells. For these reasons, this peptide has been used in the management of type 2 diabetes mellitus (DM) and has also been tried in type 1 DM. The action of GLP-1 on β cells is initiated through binding with a GLP-1 membrane bound receptor (GLP-1R), coupled to G protein, which leads to a rise in cyclic AMP (cAMP) and calcium levels, followed by insulin secretion. It was reported that GLP-1 induces an increase of PDX-1 gene expression levels and enhances the transcription of the insulin gene by increasing the binding of PDX-1 transcription factor to the insulin gene promotor region. We reported previously that silymarin induces both PDX-1 transcription factor and the insulin gene expression, as well as β-cell proliferation in pancreatic tissue. In this study we demonstrated that silymarin treatment to partially pancreatectomized rats increases pancreatic: GLP-1R gene expression, tissue immunoreactivity for GLP-1, densitometry of this peptide for Western blot analysis, proliferation of GLP-1 secreting cells, insulin serum concentration and a decrease in glucose serum. All these tests were performed to show the contrast with untreated pancreatectomized animals. Our results may suggest that silymarin increases the secretion of pancreatic GLP-1, and its activity. It would seem that it may be related to the increase in the PDX-1 transcription factor previously reported in silymarin treatment of partially pancreatectomized rats.

Key Words   
proliferation of pancreatic GLP-1 producing cells, GLP-1 pancreatic receptor, GLP-1R, GLP-1 pancreat

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To cite this article

Claudia Soto, Imelda Gonz?lez, Luis Raya, Ulises Carrasco, Luz Tovar , " Silymarin Induces Glucagon Like Peptide-1 Secretion And Increases Its Receptor Gene Expression In Pancreatic Tissue In Partially Pancreatectomized Rats ", International Journal of Latest Research in Science and Technology . Vol. 6, Issue 3, pp 13-19 , 2017

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