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International Journal of Latest Research in Science and Technology

DOI:10.29111/ijlrst   ISRA Impact Factor:3.35

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DETERMINATION OF NUCLEOSIDES IN SERUM BY LC-MS/MS FOLLOWING PROTEIN PRECIPITATION WITH ORGANIC SOLVENT

Research Paper Open Access

International Journal of Latest Research in Science and Technology Vol.5 Issue 4, pp 49-53,Year 2016

DETERMINATION OF NUCLEOSIDES IN SERUM BY LC-MS/MS FOLLOWING PROTEIN PRECIPITATION WITH ORGANIC SOLVENT

Sumaya Ferreira Guedes,Carolline Laurentino Mendes,Raquel AparecidaLoss

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Received : 23 August 2016; Accepted : 28 August 2016 ; Published : 31 August 2016

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Abstract

A lot of studies have been used to quantify the nucleosides in sick individuals. A liquid chromatography mass spectrometry (LC–MS) method was developed and validated for the determination of nine nucleosides in blood serum. The nucleosides were analyzed after a simple protein precipitation using iced organic solvents (acetone). The method was linear for all analytes with correlation coefficients (r) between 0.9872 and 0.9997. The lower limit of quantification (LLOQ) was 0,0050 µg/L e 1,000 µg/L with acceptable validation parameters. The recovery of nucleosides was assessed by extraction and showed good results, demonstrating that cleaning procedure by protein precipitation is fast, reliable and demand small quantities of organic solvent. This methodology can be used to evaluate the expression of nucleosides as biomarkers for different diseases including malignancies. Moreover, simultaneous determination of nucleosides in a single run and quick clean up process by protein precipitation by organic solvents makes it compatible with routine analysis in clinical diagnostics laboratories.

Key Words   
Nucleosides, cancer, tumor biomarkers, blood serum

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To cite this article

Sumaya Ferreira Guedes,Carolline Laurentino Mendes,Raquel AparecidaLoss , " Determination Of Nucleosides In Serum By Lc-ms/ms Following Protein Precipitation With Organic Solvent ", International Journal of Latest Research in Science and Technology . Vol. 5, Issue 4, pp 49-53 , 2016

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