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International Journal of Latest Research in Science and Technology

DOI:10.29111/ijlrst   ISRA Impact Factor:3.35

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IN - VITRO ANTIMICROBIAL ACTIVITY OF FRAMYCETIN AND PARAFFIN WOUNDDRESSING TO METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) ANDPSEUDOMONAS AERUGINOSA

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International Journal of Latest Research in Science and Technology Vol.5 Issue 2, pp 98-101,Year 2016

IN - VITRO ANTIMICROBIAL ACTIVITY OF FRAMYCETIN AND PARAFFIN WOUNDDRESSING TO METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) ANDPSEUDOMONAS AERUGINOSA

Yeva Rosana, Vanya Utami Tedhy, Indah Lestari

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Received : 23 April 2016; Accepted : 28 April 2016 ; Published : 30 April 2016

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Article No. 10647
Abstract

A good dressing provides good barrier against bacterial infection to promote natural wound healing. Framycetin and paraffin wound dressings have long been used to cover wounds to prevent infection. This study aims to determine in-vitro antimicrobial activity of framycetin and paraffin wound dressing to Methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa, multidrug resistant bacteria which are frequently found on superficial wound infection. In vitro study was conducted by exposing suspension of MRSA and Pseudomonas aeruginosa to framycetin and paraffin wound dressing. The suspension was diluted in ten time serial dilution. Plating on agar plates was done at exposure time of 0, ½, 2, 4, 6, and 24 hours. Antimicrobial activity of dressing is defined as its ability to inhibit bacterial growth. The result showed that framycetin wound dressing had antimicrobial activity against MRSA at ½ to 24 hours exposure time with bactericidal effect at 4, 6, and 24 hours. Its antimicrobial activity against Pseudomonas aeruginosa was shown at 4, 6, and 24 hours. Paraffin wound dressing showed antimicrobial activity to both bacteria at 4 and 24 hours with addition of 6 hours for MRSA. Antimicrobial activity of framycetin wound dressing against MRSA and Pseudomonas aeruginosa were superior to paraffin wound dressing.

Key Words   
Antimicrobial activity, Framycetin wound dressing, Methicillin-resistant Staphylococcus aure

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References
  1. Singal, A., Thami, G,P, "Topical antibacterial agents in dermatology", J Dermatol, Vol. 30, No.9, pp.644–6488,  Sep 2003.
  2. Brooks, G., Carroll, K., Butel, J., Morse, S. "Jawetz, Melnick, & Adelberg’s Medical Microbiology, 25th Ed", Chemical senses Atlanta: McGraw-Hill, 2010.
  3. Yolbaş, İ., Tekin, R., Keleksi, S., Selcuk, C., Okur, M., Tan, I., " Common pathogens isolated from burn wounds and their antibiotic resistance patterns",  Dicle Med J., Vol. 40, No. 3, pp.364–8, Sep 2013.
  4. Church, D., Elsayed, S., Reid, O., Lindsay, R., Winston, B., "Burn wound infections", Clin Microbiol Rev, Vol. 19, No. 2, pp.403–34, 2006.
  5. Jones, V., Grey, J.E., Harding, K.G., "ABC of Wound healing",  332, No., pp.777–780, Singer AJ, Dagum AB, 2006.
  6. Singer, A.J, Dagum A.B, "Current management of acute cutaneous wounds", N Engl J Med, Vol. 359, No.10, pp.1037–1046, Sep 2008.
  7. Kellmann, P, Lomatuell, H., "Paraffin gauze dressing , hydrophobic , sterile", 2009.
  8. Aramwit P, Muangman P, Namviriyachote N, Srichana T. In vitro evaluation of the antimicrobial effectiveness and moisture binding properties of wound dressings. Int J Mol Sci. 2010;11(8):2864–74.
  9. McPherson R, Pincus M. Henry’s Clinical Diagnosis and Management by Laboratory Methods. 22nd Ed. Saunders Elsevier; 2011.
  10. Kellmann P., " Lomatuell paraffin gauze dressing, hydrophobic, sterile", 1–3, 2009
  11. Ljungh, A., Yanagisawa, N., Wadström, T. , "Using the principle of hydrophobic interaction to bind and remove wound bacteria", J Wound Care, Vol.15, No.4, pp.175–180,
  12. Iyamba, J.M.L., Takaisi-Kikuni, N., Dulanto, S., Dehaye, J., " Study of the adhesion of clinical strains of Staphylococcus aureus on an abiotic surface using the Biofilm Ring Test®", J Biomater Nanobiotechnol, Vol. 03, No. 04, pp.547–556, 2012.
  13. Thorn, R.M.S, Greenman, J., Austin, , " In vitro method to assess the antimicrobial activity and potential efficacy of novel types of wound dressings", J Appl Microbiol., Vol.99, No.4, pp.895–90, Jan 2005.
  14. Gellatly, S.L., Hancock, R.E.W., " Pseudomonas aeruginosa: new insights into pathogenesis and host defenses", Pathog Dis., Vol. 67, No. 3, pp.159–173, Apr  
To cite this article

Yeva Rosana, Vanya Utami Tedhy, Indah Lestari , " In - Vitro Antimicrobial Activity Of Framycetin And Paraffin Wounddressing To Methicillin-resistant Staphylococcus Aureus (mrsa) Andpseudomonas Aeruginosa ", International Journal of Latest Research in Science and Technology . Vol. 5, Issue 2, pp 98-101 , 2016

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