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International Journal of Latest Research in Science and Technology

DOI:10.29111/ijlrst   ISRA Impact Factor:3.35

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i-SEARCH: DOCUMENT SEARCHING USING PAGERANK ALGORITHM

Research Paper Open Access

International Journal of Latest Research in Science and Technology Vol.4 Issue 1, pp 70-74,Year 2015

I-SEARCH: DOCUMENT SEARCHING USING PAGERANK ALGORITHM

Renalyn C. Antonio, Lorena W. Rabago,Bartolome T. Tanguilig III

Correspondence should be addressed to :

Received : 17 February 2015; Accepted : 23 February 2015 ; Published : 28 February 2015

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Article No. 10464
Abstract

Investigation on the influence of extra-cellular matrix components and organic detergents, on the direction of cell in vitro-differentiation from common precursors. The main goal was directed to searching and investigation, of mechanisms for activation of normal cell differentiation, with suppression of malignant cell transformation, at the same time, by development of ways for safe application, in particular, to the role of components from Cul-Nedd-, STAT-, and NF-κB-mediated cascade regulatory pathways in these processes. Additional copy of oncogene Dcn1 was inserted in normal mouse ESCs, for eventual elongation of life, it was important preserved normal/non-malignant characteristics to be tested and proved. On the other hand, additional copy of tumor-suppressor gene was inserted in malignant cells, for eventual decrease of their malignant potential. Fibroblasts from 3T3 cell line, derived from Balb/c mouse embryos, were propagated. One part of them was pre-incubated in cultural fluid, in which mouse malignant myeloma cells were previously cultivated, for a period of 2-3 days. After freezing of separate sub-populations from the so incubated cells for 2-4 weeks in the presence of cryo-protector Dymethylsulfoxide (DMSO), subsequent thawing and re-incubation, appearance of osteoclast-like cells was observed. On the other hand, signs of osteoblast lineage differentiation of from the derived of 3T3 cells, incubated separately for a long-term period in the presence of cultural fluid, supplemented after previous cultivation of malignant mouse myeloma cells in it, were noted.

Key Words   
Cell differentiation, Cell progenitors, Extra-cellular matrix components, Organic

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References
  1. Ali SK, Kasper SJ, Arai T, DeCaprio JA: Cul7/p185/p193 binding to Simian Virus 40 large T antigen has a role in cellular transformation. J Virol 2004, 78(6):2749-2757.
  2. Calvani N, Cafforio P, Silvestris F, Dammacco F: Functional osteoclast-like transformation of cultured human myeloma cell lines. British J Hematol 2005, 130:926-938.
  3. Cloutier A, Ear T, Blais-Charron E, Dubois CM, McDonald PP: Differentiation involvement of NF-κB and MAP kinase pathways in the generation of inflammatory cytokine by human neutrophils. J Leuk Biol 2007, 781:567-577.
  4. Cody JJ, Rivera AA, Liu J, Liu JM, Douglas JT, Feng X: A simplified method for the generation of human osteoclasts in vitro. Int J Biochem Mol Biol 2011, 2(2):183-189.
  5. Graham CH, Hawley TS, Hawley RG, MacDougall JR, Kerbel RS, Khoo N, Lala PK: Establishment and characterization of first trimester human trophoblast cells with expanded lifespan. Exp Cell Res 1993, 206:204-211.
  6. Hughes FJ, Turner W, Belibasakis G, Martuscelli G: Effects of growth factors and cytokines on osteoblast differentiation. Periodontology 2006, 2000(41):48-72.
  7. Jacob AL, Smith C, Partanen J, Ornitz DM: Fibroblast growth factor receptor 1 signaling in the osteo-chondrogenic cell lineage regulates sequential steps of osteoblast maturation. Dev Biol 2006, 296:315-328.
  8. Jahngen-Hodge J, Obin MS, Gong X, Shang S, Nowel TR, Gong J, Abasi H, Blumberg J, Taylor A: Regulation of ubiquitin-conjugating enzymes by Glutathione following oxidative stress. J Biol Chem 1997, 272:28218-28226.
  9. Jaumot M, Grana X, Giordano A, Reddy PV, Agell N, Bachs O: Cyclin/Cdk2 complexes in the nucleud of HeLa cells. Biochem Biophys Res Commun 1994, 203(3):1527-1534.
  10. Jin J, Arias EE, Chen J, Harper JW, Walter JC: A family of diverse Cul4-Dbd1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1. Mol Cell 2006, 23:709-721.
  11. Kung Sutherland MS, Lipps SG, Patnaik N, Gayo-Fung LM, Khammungkune S, Xie W, Brady HA, Barbosa MS, Anderson DW, Stein B: SP500263, a novel SERM, blocks osteoclastogenesis in a human bone cell model: role of IL-6 and GM-CSF. Cytokine 2003, 23(1-2):1-14.
  12. Kurz T, Chou YC, Willems AR, Meyer-Schaller N, Hecht ML, Tyers M, Peter M, Sicheri F: Dcn1 functions as a scaffold-type E3 ligase for cullin neddylation. Mol Cell 2008, 29:23-35.
  13. Kurz T, Ozlu N, Rudolf F, O’Rourke SM, Luke B, Hofmann K, Hyman AA, Bowerman B, Peter M: The conversed protein DCN-1/Dcn1p is required for cullin neddylation in elegans and S. cerevisiae. Nature 2005, 435:1257-1261.
  14. Kyba M, Perlingeiro RCR, Hoover RR, Lu C-W, Pierce J, Daley GQ: Enhanced hematopoietic differentiation of embryonic stem cells conditionally expressing Stat5. Proc Nat. Acad Sci USA 2003, 100:11904-11910.
  15. Lisingnoli G, Remiddi G, Cattini L, Cocchini B, Zini N, Fini M, Grassi F, Piacentini A, Facchini A: An elevated number of differentiated osteoblast colonies can be obtained from rat bone marrow stromal cells using a gradient isolation procedure. Connect Tis Res 2001, 42(1):49-58.
  16. Ma T, Shi T, Huang J, Wu L, Hu F, He PF, Deng WW, Gao P, Zhang Y, Song Q, Ma D, Qiu X: DCUN1D3, a novel UVC-responsive gene that is involved in cell cycle progression and cell growth. Mol Cell 2008, 99:2128-2135.
  17. Manandhar G, Onishchenko GE: Centriolar cycle of fused cells. J Cell Sci 1995, 108:667-673.
  18. McDonnald PP, Bald A, Cassatella MA: Activation of NF-kappaB pathway by inflammatory stimuli in human neutrophils. Blood 1997, 89(9):3421-3433.
  19. Meister A: Selective modification of glutathione metabolism. Science 1983, 220:472-477.
  20. Meyer-Schaller N, Chou Y-C, Sumara I, Martin DDO, Kurz T, Katheder N, Hofmann K, Berthiaume LG, Sicheri F, Peter M: The human Dcn1-like protein DCNL3 promotes Cul3 neddylation at membranes. Proc Natl Acad Sci USA 2009, 106(30):12365-12370.
  21. Molofsky AV, Pardal R, Morrison SJ: Diverse mechanisms regulate stem cell self-renewal. Curr Opin Cell Biol 2004, 16:700-707.
  22. Moore KJ, Fabunmi RP, Andersson LP, Freeman EW: In vitro-differentiated embryonic stem cell macrophages: a model system for studying atherosclerosis-associated macrophage functions. J Am Heart Assoc 1998, 18:1647-1654.
  23. Norwood TH, Ceigler CJ, Martin GM: Dimethyl sulfoxide enchances polyethylene glycol-mediated somatic cell fusion. Somat Cell Genet 1976, 2(3):263-270.
  24. Oakata K, Hisanaga S, Bulinski JC, Murofushi H, Aizawa H, Itoh TJ, Hotani H, Okumura E, Tachibana K, Kishimoto T: Cyclin B interaction with microtubule-associated protein 4 (MAP4) targets p34cdc2 kinase to microtubules and is a potential regulator of M-phase microtubule dynamics. J Cell Biol 1995, 128(5):849-862.
  25. O-charoenrat P, Sarkaria I, Talbot SG, Reddy P, Dao S, Ngai I, Shaha A, Kraus D, Shah J, Rusch V, Ramanathan Y, Singh B: SCCRO (DCUN1D1) induces extracellular matrix invasion by activating matrix metalloproteinase 2. Clin Cancer Res 2008, 14:6780-6789.
  26. Okuyama H, Tsuneto M, Yamane T, Yamazaki H, Hayashi S-I: Descrete types of osteoclast precursors can be generated from embryonic stem cells. Stem Cells 2003, 21:670-680.
  27. Orlandini SZ, Formigli L, Benvenuti S, Lasagni L, Franchi A, Masi L, Bernabei PA, Santini V, Brandi ML: Functional and structural interactions between osteoblastic and preosteoclastic cells in vitro. Cell and Tissue Res 1991, 281(1):33-42.
  28. Ortega AL, Mena S, Estrela JM: Glutathione in cancer cell death. Cancers 2011, 3:1285-1310.
  29. Sainova I, Kril A, Simeonov K, Popova T, Ivanov I: Investigation of the morphology of cell clones, derived from the mammalian EBTr cell line and their susceptibility to the vaccine avian poxvirus strains FK and Dessau. J Virol Meth 2005, 127(1-2):37-40.
  30. Sainova I, Valkova I, Pavlova V, Nikolova E: Experimental rodent and human models for balanced activity of oncogenes and tumor-suppressor genes and their protein products. Adv Biores 2013, 4(2):7-12.
  31. Sainova I, Vavrek I, Pavlova V, Iliev I, Yossifova L, Gardeva E, Nikolova E: Experimental model for safe in vitro- and in vivo-influence of internal and external factors of cell differentiation. Afr J Pharm Pharmacol 2011, 5(6):786-791.
  32. Solari F, Flamant F, Cherel Y, Wyers M, Jurdic P: The osteoclast generation: an in vitro and in vivo study with a genetically labelled avian monocyte cell line. J Cell Sci 1996, 109:1203-1213.
  33. Southern PJ, Berg P: Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J Mol Appl Genet 1982, 1:327-341.
  34. Susa M, Luong-Nguyen N-H, Cappellen D, Zamurovic N, Gamse R: Human primary osteoclasts:
  35. in vitro generation and applications as pharmacological and clinical assay. J Translat Med 2004, 2(1):6.
  36. Suzuki F, Saito A, Ishi K, Yamanaka K, Maruyama T, Fukunaga M, Matsumoto M, Eguchi M, Hosokawa Y, Suda K: Osteoclast-like giant cell tumor of the pancreas: an immunohistochemical and ultrastructural study. Med Electron Microsc 1997, 30:216-220.
  37. Takao T, Asanoma K, Kato K, Fukushima K, Tsunematsu R, Hirakawa T, Matsumura S, Seki H, Takeda S, Wake N: Isolation and characterization of human trophoblast side population (SP) cells in primary villous cytotrophoblasts and HTR8/SVneo cell line. PLoS ONE 2011, 6(7):e21990.
  38. Torregianni E, Lisignoli G, Manferdini C, Lambertini E, Penolazzi L, Vacchiatini R, Chieco P, Facchini A, Gambari R, Piva R: Role of Slug transcription factor in human mesenchymal stem cells. J Cell Mol Med 2012, 16(4):740-751.
  39. Vogelstein B, Kinzler KW: Cancer genes and pathways they control. Nat Med 2004, 10:789-799.
  40. Yu F, Cui Y, Zhou X, Zhang X, Han J: Osteogenic differentiation of human ligament fibroblasts induced by conditioned medium of osteoclast-like cells. BioSci Trends 2011, 5(2):46-51.
  41. Zhang L, Anglesio MS, O’sullivan M, Zhang F, Yang G, Sarao R, Ngheim MP, Cronin S, Hara H, Melnyk N, Li L, Wada T, Liu PP, Farrar J, Arceci RJ, Sorensen PH, Penninger JM: The E3 ligase HACE1 is a critical chromosome 6q21 tumor suppressor involved in multiple cancers. Nat Med 2007, 13:1060-1069.
  42. Zhang WJ, Park C, Arentson E, Choi K: Modulation of hematopoietic and endothelial cell differentiation from mouse embryonic stem cells by different culture conditions. Blood 2005, 105:111-114.
  43. Zhu H, Ji J, Shen J: Osteoblast growth promotion by protein electrostatic self-assembly on biodegradable poly(lactide). J Biomater Sci Polymer Edn 2005, 16(6):761-774.
To cite this article

Renalyn C. Antonio, Lorena W. Rabago,Bartolome T. Tanguilig III , " I-search: Document Searching Using Pagerank Algorithm ", International Journal of Latest Research in Science and Technology . Vol. 4, Issue 1, pp 70-74 , 2015

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