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International Journal of Latest Research in Science and Technology

DOI:10.29111/ijlrst   ISRA Impact Factor:3.35

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FATTY ACIDS AS AN ALTERNATIVE CARBON SOURCE FOR MCL- POLYHYDROXYALKANOATE PRODUCTION BY PSEUDOMONAS FLUORESCENS SC4

Research Paper Open Access

International Journal of Latest Research in Science and Technology Vol.4 Issue 1, pp 7-12,Year 2015

FATTY ACIDS AS AN ALTERNATIVE CARBON SOURCE FOR MCL- POLYHYDROXYALKANOATE PRODUCTION BY PSEUDOMONAS FLUORESCENS SC4

Joao Snoei,Luong Dang Nguyen,Jukka Seppala

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Received : 15 January 2015; Accepted : 03 February 2015 ; Published : 28 February 2015

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Abstract

Polyhydroxyalkanoates are biodegradable and biocompatible thermoplastics that are produced by bacteria. In this study, the capability of Pseudomonas fluorescens SC4 to produce polyhydroxyalkanoates was investigated using different fatty acids as sole carbon sources. High yield production of polyhydroxyalkanoate (PHA) by Pseudomonas fluorescens SC4 from alternative carbon sources was achieved. Linoleic acid induced the highest (44.2 wt%) PHA accumulation in bacterial cells into flask fermentation, and (45.2 wt%) in batch Fermentor at 48 h. The biopolymer recovered from fermentation was identified by NMR as medium-chain-length PHA. The strain was also able to produce biopolymer using oleic acid, however at lower concentration (31.9 wt%). No reasonable PHA was recovered from bacterial cells using caprylic acid, capric acid, undecylenic acid and sun flower oil as nutrient sources. Our experiments demonstrated that Pseudomonas fluorescens SC4 is a suitable candidate for conversion of fatty acids into value-added products as polyhydroxyalkanoates, as well as fatty acids may be considered an alternative cheap material for the production of mcl-PHA with a high productivity.

Key Words   
Fatty acids, mcl-Polyhydroxyalkanoate, Pseudomonas fluorescens, Biopolymers

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References
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To cite this article

Joao Snoei,Luong Dang Nguyen,Jukka Seppala , " Fatty Acids As An Alternative Carbon Source For Mcl- Polyhydroxyalkanoate Production By Pseudomonas Fluorescens Sc4 ", International Journal of Latest Research in Science and Technology . Vol. 4, Issue 1, pp 7-12 , 2015

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