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eISSN:2278-5299

International Journal of Latest Research in Science and Technology

DOI:10.29111/ijlrst   ISRA Impact Factor:3.35

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STRUCTURAL EVIDENCE OF RECEPTOR MEDIATED ENDOCYTOSIS AT POSTERIOR LENS SUTURES

Research Paper Open Access

International Journal of Latest Research in Science and Technology Vol.3 Issue 6, pp 68-73,Year 2014

STRUCTURAL EVIDENCE OF RECEPTOR MEDIATED ENDOCYTOSIS AT POSTERIOR LENS SUTURES

Kristin J. Al-Ghoul,Sean T. Donohue

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Received : 17 December 2014; Accepted : 25 December 2014 ; Published : 31 December 2014

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Abstract

Purpose: To investigate whether lens sutures have structural parameters (clathrin coated vesicles) to support enriched transport of metabolites into the lens via receptor mediated endocytosis. Methods: Lenses from 20 normal 6 week old rats and 6 adult chickens were utilized for this study. A transmission electron microscopic (TEM) survey was conducted to establish the presence of coated vesicles/pits within suture planes and at apical and basal ends of elongating fibers. Coated vesicle density was quantified both within and outside sutural domains. Clathrin was localized by fluorescent immunolabeling and laser scanning confocal microscopic (LSCM) visualization in both rat and chicken lenses. Results: TEM examination demonstrated that abundant coated vesicles were present at the epithelial-fiber interface (EFI) in the apical domains of both epithelial cells and elongating fibers. Quantification revealed that although coated vesicle density was greater within than without anterior sutural domains, the difference was not significant. Abundant coated vesicles were also found at basal ends of elongating fibers, however their density was significantly greater within posterior sutural domains as compared to off-suture locations (p<0.001). Statistical comparisons of anterior vs. posterior locations showed that the density of coated vesicles was significantly different in both on- and off-suture locations. Immunolocalization demonstrated that clathrin was abundant at both anterior and posterior fiber ends in elongating fibers. Clathrin immunofluorescence was more pronounced at posterior sutures than at anterior sutures. This distribution was consistent in both rat and chicken lenses. Conclusions: Posterior lens sutures appear to facilitate transport of metabolites from the vitreous into lens fibers. Although suture planes are detrimental to lens optics, their anatomical arrangement along the visual axis may be important for optimal lens function. The lower amount of clathrin at anterior as opposed to posterior sutures is probably a result of the high transport capability of the anterior lens epithelium.

Key Words   
Crystalline Lens, Clathrin, Coated Vesicles, Receptor Mediated Endocytosis, Immunohistoche

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To cite this article

Kristin J. Al-Ghoul,Sean T. Donohue , " Structural Evidence Of Receptor Mediated Endocytosis At Posterior Lens Sutures ", International Journal of Latest Research in Science and Technology . Vol. 3, Issue 6, pp 68-73 , 2014

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