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International Journal of Latest Research in Science and Technology

DOI:10.29111/ijlrst   ISRA Impact Factor:3.35

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ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF THE RED ALGA GRACILARIA VERRUCOSA FROM THE NORTH COAST OF JAVA, SEMARANG, INDONESIA

Research Paper Open Access

International Journal of Latest Research in Science and Technology Vol.3 Issue 3, pp 179-185,Year 2014

ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF THE RED ALGA GRACILARIA VERRUCOSA FROM THE NORTH COAST OF JAVA, SEMARANG, INDONESIA

Ita WIDOWATI, Daphne LUBAC,Maya PUSPITA , Nathalie BOURGOUGNON

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Received : 26 June 2014; Accepted : 28 June 2014 ; Published : 30 June 2014

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Abstract

Marine algae and bacteria are inexhaustible sources of chemical compounds that produce a wide variety of biologically active secondary metabolites, an important target for pharmaceutical industries. They contain bioactive compounds that play an important role against various diseases and against the aging process because they protect cells from oxidation. The purpose of this research is to analyze the potential antibacterial properties of the red alga Gracilaria verrucosa and its associated surface bacteria against six bacterial strains Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Vibrio harveyi, Vibrio parahaemolyticus and Vibrio alginolyticus. And also the antioxidant capacity of the algae by inhibiting the 2,2-diphenyl-1-picrylhydrazyl (DPPH). The extracts obtained were analyzed qualitatively by phytochemical screening. The total phenolic was determined by Folin-Ciocalteu reagent. Result showed that the methanol extract was the most active. It was active in 4 out of 6 strains objects antibacterial tests. The ethyl acetate extract, however, showed antioxidant activity (EC50 = 173 ppm) supported by the phytochemical study of the compounds of the extract. Content and the presence of phenolic compounds (14.58 mg Gallic acid equivalent / g of dry sample) and flavonoids (presence) known for their antioxidant effects were in accordance with the data obtained. The phytochemical study showed that G. verrucosa contained many bioactive compounds with diverse biological properties. Symbiotic bacteria isolated from the alga showed no antibacterial activity.

Key Words   
Antibacterial, Antioxidant, Red Algae, Gracilaria verrucosa

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To cite this article

Ita WIDOWATI, Daphne LUBAC,Maya PUSPITA , Nathalie BOURGOUGNON , " Antibacterial And Antioxidant Properties Of The Red Alga Gracilaria Verrucosa From The North Coast Of Java, Semarang, Indonesia ", International Journal of Latest Research in Science and Technology . Vol. 3, Issue 3, pp 179-185 , 2014

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